Autoradiographic demonstration of ocular-dominance columns in the monkey striate cortex by means of transneuronal transport.

摘要: In the past few years technique of mapping pathways in central nervous system by anterograde axoplasmic transport radioactive molecules has come into wide use and is now an important supplement to Nauta degeneration methods e,6,1°,13. Several investigatorsa,3,v,9,12 have noted substances postsynaptic ceils, which suggests that these cells take up labeled material released from terminals 1,3,7. Grafstein 4 was first explore possibility tracing a pathway beyond initially cells, examining also projections recipient neurons. By radiochemical measurements autoradiography, it shown after injection [3H]proline [aH]fucose one eye mouse contralateral striate cortex more heavily than ipsilateral, label concentrated layer IV 5,14. macaque monkey geniculostriate terminates very dense, highly localized manner, mainly C. Furthermore, 2 eyes end characteristic alternating stripe-like pattern oculardominance columns s. It occurred us if were transported transneuronally, followed autoradiography might reveal entire ocular-dominance columns. For autoradiographic study transneuronal primate visual system, 50/~1 saline solution containing L-[6-3H]fucose (2.5 mCi/ml, 13.4Ci/ mmole) L-[3H]proline (7.5 generally labeled, 6.8 Ci/mmole) injected vitreous left ofa 3 kg normal Rhesus macaque. This repeated 5 times at 12 h intervals (total dose 3.0 mCi). The animal perfused with 10 ~ formalin weeks initial injection. lateral geniculate nucleus cut 20 #m frozen sections, embedded paraffin 15/~m sections. sections coated Ilford K5 emulsion, dark for 2-4 months developed Dektol. Sections counter-stained thionin. all layers receiving strongly (Fig. l A B). other showed grain counts higher background, perhaps partly because fibers passage retina,