Spin trapping of free radicals during hepatic microsomal lipid peroxidation

摘要: Abstract We have used spin-trapping techniques to identify radical species formed during the NADPH-stimulated peroxidation of rat hepatic microsomes. Using 5,5-dimethyl-1-pyrroline-1-oxide, we confirmed presence substantial quantities superoxide but found evidence for formation only small hydroxyl radical. Use spin traps N-tert-butyl-alpha-4-pyridylnitrone-1-oxide and 2-methyl-2-nitroso-1-propanol allowed us determine that lipid peroxyl radicals are predominant "lipid-type" in peroxidizing microsomes under aerobic conditions. These data suggest microsomal dienyl react with molecular oxygen at near diffusion-controlled rates.