Method for selecting a single cell expressing a heterogeneous combination of antibodies
作者： Ton Logtenberg , Hendricus Renerus Jacobus Mattheus Hoogenboom
DOI:
关键词:
摘要: The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components the combination being functional and compatible with each other. further a method for producing composition comprising at least two different proteinaceous molecules paired variable regions, having specificities, contacting three regions under conditions allowing pairing harvesting specificities resulting from pairing.
google.com.tr参考文章(582)
Helmut Lenz, Ulrich H. Weidle, Expression of heterobispecific antibodies by genes transfected into producer hybridoma cells Gene. ,vol. 87, pp. 213- 218 ,(1990) , 10.1016/0378-1119(90)90304-A
Melvyn Little, Frank Breitling, Stefan Dübel, Patrick Fuchs, Michael Braunagel, Human antibody libraries in Escherichia coli Journal of Biotechnology. ,vol. 41, pp. 187- 195 ,(1995) , 10.1016/0168-1656(95)00022-I
N Bhardwaj, A Bender, N Gonzalez, L K Bui, M C Garrett, R M Steinman, Influenza virus-infected dendritic cells stimulate strong proliferative and cytolytic responses from human CD8+ T cells. Journal of Clinical Investigation. ,vol. 94, pp. 797- 807 ,(1994) , 10.1172/JCI117399
Jay S. Fine, Heather D. Macosko, Michael J. Grace, Satwant K. Narula, Interleukin-10 Enhances γδ T Cell Development in the Murine Fetal Thymus Cellular Immunology. ,vol. 155, pp. 111- 122 ,(1994) , 10.1006/CIMM.1994.1105
Yakov Gluzman, SV40-transformed simian cells support the replication of early SV40 mutants Cell. ,vol. 23, pp. 175- 182 ,(1981) , 10.1016/0092-8674(81)90282-8
Lisa J. Garrard, Maria Yang, Mark P. O'Connell, Robert F. Kelley, Dennis J. Henner, FAB Assembly and Enrichment in a Monovalent Phage Display System Nature Biotechnology. ,vol. 9, pp. 1373- 1377 ,(1991) , 10.1038/NBT1291-1373
Yolanda S Lie, Christos J Petropoulos, Advances in quantitative PCR technology: 5' nuclease assays. Current Opinion in Biotechnology. ,vol. 9, pp. 43- 48 ,(1998) , 10.1016/S0958-1669(98)80082-7
John de Kruif, Edwin Boel, Ton Logtenberg, Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions. Journal of Molecular Biology. ,vol. 248, pp. 97- 105 ,(1995) , 10.1006/JMBI.1995.0204
Steffen Schlehuber, Arne Skerra, Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. Biophysical Chemistry. ,vol. 96, pp. 213- 228 ,(2002) , 10.1016/S0301-4622(02)00026-1
Franck Toledo, Chung-Wen Liu, Crystal J Lee, Geoffrey M Wahl, None, RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses Nucleic Acids Research. ,vol. 34, ,(2006) , 10.1093/NAR/GKL518