Immobilization of graphene-derived materials at gold surfaces: Towards a rational design of protein-based platforms for electrochemical and plasmonic applications

摘要: Abstract This work is focused on the critical analysis of non-covalent modification a thiolated-gold surface with different grapheneous materials and covalent attachment bovine serum albumin (BSA) as model protein. The main goal was to find relationship between nature amount nanomaterial, immobilized protein, electrochemical plasmonic properties resulting platforms. characterization nanomaterials (graphene oxide (GO), GO modified chitosan (CHIT), (GO-CHIT), chemically reduced GO-CHIT (RGO-CHIT)) performed by using FTIR, Raman, TGA, Dynamic light scattering (DLS), UV–Vis spectroscopy ζ-potential measurements. surfaces BSA plasmon resonance (SPR), cyclic voltammetry, impedance (EIS) scanning microscopy (SECM). pH dispersions demonstrated be parameter control assembly protein at gold and, consequently, electroactivity plasmonics When GO, optimum 8.00 while in case RGO-CHIT, pHs  CHIT are most adequate. We that our system, if detection method depends direct quantification platform (like SPR), use best option; mode changes response redox marker EIS), selected material should RGO.