Caseicin 80: purification and characterization of a new bacteriocin from Lactobacillus casei.

摘要: When grown in complex or synthetic media, Lactobacillus casei B 80 synthesizes a mitomycin C-inducible polypeptide with very specific bactericidal activity against the sensitive strain 109. The amount of secreted bacteriocin culture solution was low, about 1 mg/l. which we called caseicin 80, also detectable cell extracts, although only 2% total retained intracellularly. Caseicin concentrated by ultrafiltration and purified cation exchange chromatography Cellulose SE-23 Superose. molecular weight range M r=40,000–42,000 isoelectric point pH 4.5.