Determination of the oxidative burst chemiluminescent response of avian and murine-derived macrophages versus corresponding cell lines in relation to stimulation with Salmonella serotypes

摘要: Abstract In contrast to mammalian systems, avian species lack a resident or harvestable macrophage population in the abdominal exudate. Peritoneal macrophages chicken can be elicited if an inflammatory agent such as sephadex is injected. This study examines kinetics of different populations, derived by methods isolation and from hosts, with respect oxidative burst upon infection host-adapted Salmonella serotypes. The nature murine avian-derived cell line was determined after stimulation phorbol myristate (PMA), zymosan A, Both peritoneal macrophages, blood monocytes corresponding lines, J774A.1 HD-11, were unable produce detectable chemiluminescent (CL) response interaction using luminescent probe luminol. However, both PMA A induced CL all types, eliciting higher earlier peak (pkH) than A. Lucigenin-enhanced achieved PMA, this case, responses PMA. there no significant differences (murine) cells demonstrated differences, S. enterica serotype Choleraesuis (S. choleraesuis gallinarum producing highest response. HD-11 (chicken) cells, dublin CL. With abortusovis failed induce appreciable these experiments it that not monocytes/macrophage populations luminol, which suggests link myeloperoxidase system cells. appeared independent system, indicating production another compared No discernable effect host specificity regard respective seen host-derived displayed altered functional characteristics comparison their primary counterparts.