Lack ofMycobacterium tuberculosis-specific interleukin-17A-producing CD4+T cells in active disease

摘要: Protective immunity to Mycobacterium tuberculosis (Mtb) is commonly ascribed a Th1 profile; however, the involvement of Th17 cells remains be clarified. Here, we characterized Mtb-specific CD4+ T cells in blood and bronchoalveolar lavages (BALs) from untreated subjects with either active disease (TB) or latent Mtb infection (LTBI), considered as prototypic models uncontrolled controlled infection, respectively. The production IL-17A, IFN-γ, TNF-α, IL-2 by was assessed both directly ex vivo following vitro antigen-specific T-cell expansion. Unlike for extracellular bacteria, responses lacked immediate IL-17A effector function LTBI TB individuals. Furthermore, were absent BALs, while bacteria-specific identified gut biopsies healthy Interestingly, only 50% but not acquired ability produce Finally, acquisition correlated coexpression CXCR3 CCR6, currently associated profiles, Our data demonstrate that are selectively undetectable peripheral BALs patients.