Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase.

摘要: We have previously reported the cloning of cDNAs coding for two isoforms (termed C alpha and beta) catalytic (C) subunit cAMP-dependent protein kinase (Uhler, M., Chrivia, J., McKnight, G. S. (1986) J. Biol. Chem. 261, 15360-15363). constructed cDNA expression vectors beta proteins using mouse metallothionein promoter so that mRNA transcripts from are inducible with Zn. In stable transformants NIH 3T3 AtT-20 cells, induction both Zn increases as well cAMP-independent activity. The synthesized these can be detected by Western blot analysis slightly different molecular weights determined sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Interestingly, levels RI also shown to increase in cells expressing high or subunits although there is no change mRNA. contrast, RII not significantly affected increasing either cell lines. conclude compensate increased a corresponding elevation implying interact form holoenzyme. relevance experimental results coordinate regulation discussed.