Chemical characterization of the membrane-anchoring domain of human placental alkaline phosphatase.
作者： S Ogata , Y Hayashi , N Takami , Y Ikehara
DOI: 10.1016/S0021-9258(19)81542-9
关键词:
摘要: Membrane and soluble forms of alkaline phosphatase (ALP) were selectively prepared from human placental microsomes by treatment with 1-butanol at pH 8.5 5.5, respectively. The purified membrane (mALP) (sALP) analyzed for chemical compositions. mALP was found to contain 1 mol each palmitate, stearate, glycerol/subunit ALP, which absent in sALP. Both the contained inositol 2 ethanolamine/subunit. However, none these compounds detectable another form papain, is known cleave carboxyl-terminal region. results suggest that contains diacylglycerol, removal its conversion We then [3H]ethanolamine-labeled ALP incubating choriocarcinoma cells (JEG-3) isotope. 3H-Labeled sALP mixed unlabeled treated papain. A 3H-labeled single component digests sequential chromatography through anti-ALP-IgG-Sepharose, concanavalin A-Sepharose, Bio-Gel P-6, TSK G-2000 columns. Chemical analyses revealed sample tripeptide Thr-Thr-Asp, ethanolamine, glucosamine, mannose, inositol, phosphate. Molar ratios latter five calculated be 2, 1, 3, respectively, taking Asp as mol. sequence identified positions 482-484 primary structure deduced cDNA sequence, predicts a further extension position 513, containing hydrophobic amino acid sequence. Taken together, mature molecule lacks predicted peptide attached Asp484 glycosylphospholipid, components are characterized above. glycosylphospholipid thus considered function anchor ALP.
sci-hub.st 参考文章(43)
M A Ferguson, G A Cross, Myristylation of the membrane form of a Trypanosoma brucei variant surface glycoprotein. Journal of Biological Chemistry. ,vol. 259, pp. 3011- 3015 ,(1984) , 10.1016/S0021-9258(17)43250-9
TOSHIO IMANARI, YASUAKI ARAKAWA, ZENZO TAMURA, Gas Chromatographic Analysis of Aldoses Chemical & Pharmaceutical Bulletin. ,vol. 17, pp. 1967- 1969 ,(1969) , 10.1248/CPB.17.1967
Milton W. Slein, Gerald F. Logan, Characterization of the Phospholipases of Bacillus cereus and Their Effects on Erythrocytes, Bone, and Kidney Cells Journal of Bacteriology. ,vol. 90, pp. 69- 81 ,(1965) , 10.1128/JB.90.1.69-81.1965
Akira MIKI, Yukari TANAKA, Shigenori OGATA, Yukio IKEHARA, Selective preparation and characterization of membranous and soluble forms of alkaline phosphatase from rat tissues FEBS Journal. ,vol. 160, pp. 41- 48 ,(1986) , 10.1111/J.1432-1033.1986.TB09937.X
M A Ferguson, M G Low, G A Cross, Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein. Journal of Biological Chemistry. ,vol. 260, pp. 14547- 14555 ,(1985) , 10.1016/S0021-9258(17)38603-9
J L Millán, Molecular cloning and sequence analysis of human placental alkaline phosphatase. Journal of Biological Chemistry. ,vol. 261, pp. 3112- 3115 ,(1986) , 10.1016/S0021-9258(17)35755-1
Yukio IKEHARA, Mihoko MIYASATO, Shigenori OGATA, Kimimitsu ODA, Multiple Forms of Rat‐Serum α1‐Protease Inhibitor FEBS Journal. ,vol. 115, pp. 253- 260 ,(1981) , 10.1111/J.1432-1033.1981.TB05231.X
Ikezawa Hiroh, Yamanegi Masato, Taguchi Ryo, Miyashita Tomoyuki, Ohyabu Tetsuo, Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 450, pp. 154- 164 ,(1976) , 10.1016/0005-2760(76)90087-4
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
M G Low, Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors. Biochemical Journal. ,vol. 244, pp. 1- 13 ,(1987) , 10.1042/BJ2440001