Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.
作者： M A Ferguson , M G Low , G A Cross
DOI: 10.1016/S0021-9258(17)38603-9
关键词:
摘要: The COOH terminus of the externally disposed variant surface glycoprotein (VSG) eukaryotic pathogenic protozoan Trypanosoma brucei strain 427 MITat 1.4 (117) is covalently linked to a novel phosphatidylinositol-containing glycolipid. This conclusion supported by analysis products nitrous acid deamination or Staphylococcus aureus phosphatidylinositol-specific phospholipase C treatment purified membrane-form VSG. Lysis trypanosomes accompanied release soluble VSG, catalyzed activation an endogenous C. only apparent difference between VSG and removal sn-1,2-dimyristylglycerol. COOH-terminal glycopeptide derived Pronase digestion was characterized chemical modification with alkaline phosphatase. results are consistent single non-N-acetylated glucosamine residue being reducing oligosaccharide in glycosidic linkage myo-inositol monophosphate that probably 1,2-cyclic monophosphate. A partial structure for moiety presented. represents new type post-translational protein membrane anchor. We discuss relevance this observations have been made other proteins.
sci-hub.st 参考文章(57)
D G Campbell, J Gagnon, K B Reid, A F Williams, Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region Biochemical Journal. ,vol. 195, pp. 15- 30 ,(1981) , 10.1042/BJ1950015
T. Baltz, C. Giroud, D. Baltz, G. Duvillier, P. Degand, J. Demaille, R. Pautrizel, The variable surface glycoproteins of Trypanosoma equiperdum are phosphorylated. The EMBO Journal. ,vol. 1, pp. 1393- 1398 ,(1982) , 10.1002/J.1460-2075.1982.TB01328.X
A. A. Holder, Glycosylation of the variant surface antigens of Trypanosoma brucei. Current Topics in Microbiology and Immunology. ,vol. 117, pp. 57- 74 ,(1985) , 10.1007/978-3-642-70538-0_3
Michael F. G. Schmidt, Fatty acid binding: a new kind of posttranslational modification of membrane proteins. Current Topics in Microbiology and Immunology. ,vol. 102, pp. 101- 129 ,(1983) , 10.1007/978-3-642-68906-2_3
R. E. Chambers, J. R. Clamp, An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials. Biochemical Journal. ,vol. 125, pp. 1009- 1018 ,(1971) , 10.1042/BJ1251009
G. Duvillier, A. Nouvelot, C. Richet, T. Baltz, P. Degand, Presence of glycerol and fatty acids in the C-terminal end of a variant surface glycoprotein from Trypanosoma equiperdum Biochemical and Biophysical Research Communications. ,vol. 114, pp. 119- 125 ,(1983) , 10.1016/0006-291X(83)91602-9
K Haldar, M A Ferguson, G A Cross, Acylation of a Plasmodium falciparum merozoite surface antigen via sn-1,2-diacyl glycerol. Journal of Biological Chemistry. ,vol. 260, pp. 4969- 4974 ,(1985) , 10.1016/S0021-9258(18)89167-0
M A Ferguson, G A Cross, Myristylation of the membrane form of a Trypanosoma brucei variant surface glycoprotein. Journal of Biological Chemistry. ,vol. 259, pp. 3011- 3015 ,(1984) , 10.1016/S0021-9258(17)43250-9
M G Low, J B Finean, Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C. Biochemical Journal. ,vol. 167, pp. 281- 284 ,(1977) , 10.1042/BJ1670281
Grant R. Bartlett, Phosphorus Assay in Column Chromatography Journal of Biological Chemistry. ,vol. 234, pp. 466- 468 ,(1959) , 10.1016/S0021-9258(18)70226-3