Influence of cations on the blue to purple transition of bacteriorhodopsin. Comparison of Ca2+ and Hg2+ binding and their effect on the surface potential.
作者： M Duñach , M Seigneuret , J L Rigaud , E Padrós
DOI: 10.1016/S0021-9258(19)77846-6
关键词:
摘要: We have investigated the effect of Ca2+ and Hg2+ binding on various properties blue membrane prepared by deionization Halobacterium halobium purple membrane. Binding radioactive 45Ca2+ 203Hg2+ was monitored a filtration technique. Five high medium affinity sites for seven low were found per bacteriorhodopsin. Competitive observed only three Hg2+. Visible absorption studies indicated that could restore color bacteriorhodopsin while inefficient. Hg2- partially reverse to Ca2+-regenerated in parallel with displacement Ca2+. Effects cation surface potential measured Electron Spin Resonance spectroscopy using cationic spin-labeled amphiphile. Cations such as La3+, Ca2+, Mg2+, or Na+ strongly increased (i.e. rendered less negative) potential. An univocal correlation between cation-induced variation extent regeneration color. induced smaller increase than corresponding effective divalent cations. This lower appears be due not related those other
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