Solid phase cytometry as a tool to detect viable but non-culturable cells of Campylobacter jejuni.
作者： I. Cools , E. D'Haese , M. Uyttendaele , E. Storms , H.J. Nelis
DOI: 10.1016/J.MIMET.2005.02.023
关键词:
摘要: Abstract Solid phase cytometry (SPC) in conjunction with fluorescent viability staining has been investigated as a tool to detect viable but non-culturable Campylobacter jejuni drinking water. Inoculated water samples were filtered over polyester membrane filter and the retained cells stained using carboxyfluorescein ester substrate for intracellular esterases. The number of green bacteria was automatically counted by an Ar laser scanning device (ChemScan®) 3 min. In parallel, plate count determined on Columbia Blood Agar. culturable decreased below detection limit counting less than 50 days. contrast, remained at its initial level least 85 discrepancy between two results can be attributed transition C. into VBNC cells. Furthermore, SPC distinguish low numbers dividing non-dividing it potential monitor attempts resuscitate
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