Selenium-dependent glutathione peroxidase-GI is a major glutathione peroxidase activity in the mucosal epithelium of rodent intestine.

摘要: Gpx2 mRNA, encoding a selenium-dependent glutathione peroxidase (GPX-GI), has been found to be highly expressed in the gastrointestinal tract (GI) mucosal epithelium. In this study, we show that GPX-GI is produced epithelium of adult rat GI and activity levels are comparable from GPX-1. Post-mitochondrial supernatant GPX complete length small intestine was partially purified. A sample enriched for putative fractionated by SDS-polyacrylamide gel electrophoresis. Polypeptides 21 kDa 22 were digested with trypsin. After resolving tryptic peptides high pressure liquid chromatography (HPLC), major peaks analyzed their amino acid sequence Microflow-HPLC-Tandem Mass Spectrometry automated Edman degradation sequencing. Both methods revealed 21-kDa contained determined homology deduced mouse polypeptide sequence. Rat GPX-1 also detected samples. AntiGPX-GI antiGPX-1 antibodies used determine distribution respective isoenzyme activities along respect crypt villus axis rats. uniformly distributed middle lower axis. accounted nearly same percentage total as all these compartments. Studies on distal ileum segment wildtype Gpx1 gene knockout mice showed at parity segment.