Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts.
作者： Marvin Kadisch , Christian Willrodt , Michael Hillen , Bruno Bühler , Andreas Schmid
关键词:
摘要: A systematic and powerful knowledge-based framework exists for improving the activity stability of chemical catalysts empowering commercialization respective processes. In contrast, corresponding biotechnological processes are still scarce characterized by case-by-case development strategies. understanding parameters affecting biocatalyst efficiency, that is, stability, is essential a rational generation improved biocatalysts. Today, approaches only exist increasing whole-cell They largely missing stability. this review, we structure factors summarize existing, yet not completely exploited strategies to overcome limitations. The mechanisms related destabilization discussed demonstrated inter alia based on two case studies. similar with different objectives regarding target molecule or metabolic pathway complexity process scale, but in turn highly interdependent. This review provides stabilization combination our knowledge improve activity, paves way design superior recombinant biocatalysts, which can then be employed economically ecologically competitive sustainable bioprocesses.
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