Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device

作者: Nai-Chin Chen , Chun-Hong Chen , Ming-Kun Chen , Ling-Sheng Jang , Min-Haw Wang

DOI: 10.1016/J.SNB.2013.08.104

关键词:

摘要: Abstract This paper integrates fluid manipulation, single-cell trapping, and impedance measurement into a parallel-plate chip. Liquid dielectophoresis (LDEP) dielectrophoresis (DEP) are utilized to manipulate sucrose solution HeLa cells in chip, respectively. The with is transported by LDEP. A cell trapped DEP then its measured an SU-8 cavity between electrodes. Finite element analysis (FEA) used optimize the microstructure of cavity.

参考文章(21)
Thomas B. Jones, Electromechanics of Particles Cambridge University Press. ,(1995) , 10.1017/CBO9780511574498
G. A. Neil, Ulrich Zimmermann, Electromanipulation of cells ,(1996)
Ji-Wei Wang, Min-Haw Wang, Ling-Sheng Jang, Effects of electrode geometry and cell location on single-cell impedance measurement. Biosensors and Bioelectronics. ,vol. 25, pp. 1271- 1276 ,(2010) , 10.1016/J.BIOS.2009.10.015
T. B. Jones, M. Gunji, M. Washizu, M. J. Feldman, Dielectrophoretic liquid actuation and nanodroplet formation Journal of Applied Physics. ,vol. 89, pp. 1441- 1448 ,(2001) , 10.1063/1.1332799
Steven J. Altschuler, Lani F. Wu, Cellular Heterogeneity: Do Differences Make a Difference? Cell. ,vol. 141, pp. 559- 563 ,(2010) , 10.1016/J.CELL.2010.04.033
Gaurav J. Shah, Jeffrey L. Veale, Yael Korin, Elaine F. Reed, H. Albin Gritsch, Chang-Jin “CJ” Kim, Specific binding and magnetic concentration of CD8+ T-lymphocytes on electrowetting-on-dielectric platform. Biomicrofluidics. ,vol. 4, pp. 044106- ,(2010) , 10.1063/1.3509457
Herbert A. Pohl, Kent Pollock, Electrode geometries for various dielectrophoretic force laws Journal of Electrostatics. ,vol. 5, pp. 337- 342 ,(1978) , 10.1016/0304-3886(78)90028-1
Ling-Sheng Jang, Pao-Hua Huang, Kung-Chieh Lan, Single-cell trapping utilizing negative dielectrophoretic quadrupole and microwell electrodes Biosensors and Bioelectronics. ,vol. 24, pp. 3637- 3644 ,(2009) , 10.1016/J.BIOS.2009.05.027
Helene Andersson, Wouter van der Wijngaart, Peter Nilsson, Peter Enoksson, Göran Stemme, A valve-less diffuser micropump for microfluidic analytical systems Sensors and Actuators B: Chemical. ,vol. 72, pp. 259- 265 ,(2001) , 10.1016/S0925-4005(00)00644-4