Genetic Variability and Biotechnological Studies for the Conservation and Improvement of Ensete ventricosum

摘要: Enset (Ensete ventricosum) is a drought-resistant staple food crop which grown in the south and south-western parts of Ethiopia. Due to lack research on enset, no benefits have yet been gained from modern biotechnological approaches. This thesis deals with studies genetic diversity cultivated wild development micropropagation transformation procedures identification microbes associated enset. The enset nine enset-growing regions Ethiopia was investigated using Random Amplified Polymorphic DNA (RAPD). All 111 clones studied showed unique band patterns. observed particular area appears be related extent cultivation culture distribution pattern different ethnic groups than geographical distance. RAPD also applied document 5 populations together some Musa species. Both indicated that within higher among populations. Our results suggest current originated limited number progenitors. large gene pool species could utilized for improvement crop. Two focused efficient order develop optimal systems procedure developed enabled production numbers propagules. Wounding meristem modifying nutrients medium essential enhance efficiency micropropagation. In experiment, expression β-glucuronidase (gus) manifested explants two clones, both particle bombardment Agrobacterium. Agrobacterium shown infect monocot plant resulting percentage (63%) GUS (18%), when shoot tips were sonicated. frequency expressing vary depending sonication treatment, tissue type, co-culture period techniques. Microbial contamination, by apparently endophytic are resistant antimicrobial agents encountered during work. persistent problem prompted microbiological study 16 bacteria, 7 yeasts 1 unknown mycelial fungus surface-disinfected vitro field-grown 16S 26S rDNA sequences. Some identified pathogenic