A new approach to determining cholinesterase activities in samples of whole blood

摘要: Abstract A sensitive method, especially suitable for clinical laboratories, the routine determination of cholinesterase activities in whole blood is presented. This method based on hydrolysis propionylthiocholine and spectrophotometric thiocholine produced by reaction with 4,4'-dithiodipyridine. The product 4-thiopyridone has an absorption maximum at 324 nm, so that measurement presence hemoglobin possible. Propionylthiocholine used as substrate both plasma butyrylcholinesterase erythrocyte acetylcholinesterase. These two enzymes, relative amounts which they are present human blood, split this ester about same rate. Consequently, a first gives total activity each individual 50%. second selective inhibitor (“Astra 1397”) difference between values represents enzyme. validity reliability results were checked sample ways: (1) determining earlier gasometric technique uses samples dried filter paper; (2) measuring separated hemolysate substrate.