Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells.

摘要: Quantum dots (QDs), as new and promising fluorescent probes, hold great potential in long term non-invasive bio-imaging, however there are many uncovered issues regarding their competency. In the present study, different QDs (525, 585 800 nm) were used to label CD133, CD34, CD14 mesenchymal stem cells (MSCs) using positively charged peptides. Results demonstrated highly efficient internalization with possible involvement of macropinocytosis. As indicated by LDH release TUNEL assay, no measurable effects on cell viability detected at a concentration 10 nM. did not have any deleterious normal functionality where both labeled CD133(+) MSCs remarkably differentiated along multiple lineages use colony forming assay adipo/osteo induction, respectively. Our results QD maintenance revealed that these nano-particles properly stable various excretion times been observed depending particle size type. vitro co-culture system transplantation an animal model showed leaked out from released able re-enter adjacent over time. These data suggest before utilization bio-imaging related applications, intra-cellular delivery technique should be considered preserve for prolonged time well eliminating leakage.