Rabbits with elevated serum C-reactive protein exhibit diminished neutrophil infiltration and vascular permeability in C5a-induced alveolitis.

摘要: In previous studies, we have shown that C-reactive protein (CRP) inhibits chemotaxis of neutrophils to complement fragments in vitro. To evaluate the effect CRP on C5a-induced inflammation vivo, a rabbit model alveolitis was used. Rabbits pretreated with subcutaneous injections croton oil had serum increase from undetectable levels 270 +/- 70 micrograms/ml 48 hours later. were intubated and C5a des arg (10 micrograms/ml) instilled directly into lungs via an endotracheal tube. Four six later, animals killed bronchoalveolar lavage performed. significantly (P < 0.01) reduced arg-stimulated neutrophil infiltration (30 5%) alveolar air spaces compared untreated rabbits (64 9%). Increased numbers total leukocytes washes coincided increased whereas macrophages remained unchanged all groups. also significant decrease 0.05) (320 50 fluid after instillation (850 140 micrograms/ml). vitro, (50 added normal inhibited human by 41%. Finally, direct intravenous pretreatment purified alveolitis. The CRP-C5a group 33 10% infiltration, reduction (71 6%). content 986 165 fluid, which lower than (1645 363 Therefore, development leakage vivo These data indicate offers protective neutrophil-mediated lung injury reducing influx leak.