Wolbachia Interferes with Ferritin Expression and Iron Metabolism in Insects

摘要: Wolbachia is an intracellular bacterium generally described as being a facultative reproductive parasite. However, necessary for oogenesis completion in the wasp Asobara tabida. This dependence has evolved recently result of interference with apoptosis during oogenesis. Through comparative transcriptomics between symbiotic and aposymbiotic individuals, we observed differential expression ferritin, which forms complex involved iron storage. Iron essential element that limited supply cell. it also highly toxic precursor Reactive Oxygen Species (ROS). Ferritin been shown to play key role host–pathogen interactions. Measuring ferritin by quantitative RT-PCR, confirmed was upregulated compared individuals. Manipulating content diet, showed overload markedly affected development induced apoptotic processes A. tabida, suggesting regulation homeostasis may be related obligate wasp. Finally, demonstrated metabolism influenced presence not only mutualism but parasitism involving Drosophila simulans Aedes aegypti cells. In these latter cases, bacterioferritin increased iron, showing responds concentration iron. Our results indicate interfere metabolism. The high affinity might due physiological requirement bacterium, could what allows symbiont persist organism reducing labile concentration, thus protecting cell from oxidative stress apoptosis. These findings reinforce idea pathogenic, parasitic mutualistic bacteria all use same molecular mechanisms survive replicate within host By impacting general physiology host, select compensatory mechanisms, extends possible consequences persistent endosymbiont on evolution their hosts.