Applying imaging ToF-SIMS and PCA in differentiation of tissue types.
作者： Ligang Wu , James S. Felton , Kuang Jen J. Wu
DOI: 10.1007/978-1-60761-746-4_16
关键词:
摘要: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has proven to be an extremely powerful tool for characterizing chemical distributions within biological cells and tissues. However, differentiating samples, e.g., cancerous from their normal counterparts or benign tissues malignant tissues, presents unique challenges ToF-SIMS. Repeatable differentiation of such especially formalin-fixed paraffin-embedded (FFPE) histological specimens, could used improve tissue-based diagnosis aid in prognosis decisions. In this chapter, we describe a strategy FFPE ToF-SIMS was image deparaffinized mouse embryos differentiate tissue types. The robustness repeatability the method determined by analyzing ten slices three different over period 1 month. Using principal component analysis (PCA) reduce spectral data generated ToF-SIMS, histopathologically identified types can differentiated based on characteristic differences spectra.
springer.com
sci-hub.st 参考文章(22)
Ylva Magnusson, Peter Friberg, Peter Sjövall, Frida Dangardt, Per Malmberg, Yun Chen, Lipid imaging of human skeletal muscle using TOF-SIMS with bismuth cluster ion as a primary ion source. Clinical Physiology and Functional Imaging. ,vol. 28, pp. 202- 209 ,(2008) , 10.1111/J.1475-097X.2008.00796.X
Kristen S. Kulp, Elena S. F. Berman, Mark G. Knize, David L. Shattuck, Erik J. Nelson, Ligang Wu, Jennifer L. Montgomery, James S. Felton, Kuang Jen Wu, Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry. Analytical Chemistry. ,vol. 78, pp. 3651- 3658 ,(2006) , 10.1021/AC060054C
Donald M. Cannon, Michaeleen L. Pacholski, Nicholas Winograd, Andrew G. Ewing, Molecule Specific Imaging of Freeze-Fractured, Frozen-Hydrated Model Membrane Systems Using Mass Spectrometry Journal of the American Chemical Society. ,vol. 122, pp. 603- 610 ,(2000) , 10.1021/JA992078P
Chi-Ying Lee, Gregory M. Harbers, David W. Grainger, Lara J. Gamble, David G. Castner, Fluorescence, XPS, and TOF-SIMS surface chemical state image analysis of DNA microarrays. Journal of the American Chemical Society. ,vol. 129, pp. 9429- 9438 ,(2007) , 10.1021/JA071879M
A F Maarten Altelaar, Stefan L Luxembourg, Liam A McDonnell, Sander R Piersma, Ron M A Heeren, Imaging mass spectrometry at cellular length scales Nature Protocols. ,vol. 2, pp. 1185- 1196 ,(2007) , 10.1038/NPROT.2007.117
H. Jungnickel, E. A. Jones, N. P. Lockyer, S. G. Oliver, G. M. Stephens, J. C. Vickerman, Application of TOF-SIMS with chemometrics to discriminate between four different yeast strains from the species Candida glabrata and Saccharomyces cerevisiae. Analytical Chemistry. ,vol. 77, pp. 1740- 1745 ,(2005) , 10.1021/AC048792T
I.M. Kempson, W.M. Skinner, P.K. Kirkbride, Calcium distributions in human hair by ToF-SIMS. Biochimica et Biophysica Acta. ,vol. 1624, pp. 1- 5 ,(2003) , 10.1016/J.BBAGEN.2003.09.003
Elena S. F. Berman, Kristen S. Kulp, Mark G. Knize, Ligang Wu, Erik J. Nelson, David O. Nelson, Kuang Jen Wu, Distinguishing monosaccharide stereo- and structural isomers with TOF-SIMS and multivariate statistical analysis. Analytical Chemistry. ,vol. 78, pp. 6497- 6503 ,(2006) , 10.1021/AC060865G
Ligang Wu, Xiaochen Lu, Kristen S. Kulp, Mark G. Knize, Elena S.F. Berman, Erik J. Nelson, James S. Felton, Kuang Jen J. Wu, Imaging and differentiation of mouse embryo tissues by ToF-SIMS International Journal of Mass Spectrometry. ,vol. 260, pp. 137- 145 ,(2007) , 10.1016/J.IJMS.2006.09.029
K BORNER, H NYGREN, B HAGENHOFF, P MALMBERG, E TALLAREK, J MANSSON, Distribution of cholesterol and galactosylceramide in rat cerebellar white matter. Biochimica et Biophysica Acta. ,vol. 1761, pp. 335- 344 ,(2006) , 10.1016/J.BBALIP.2006.02.021