Lipid imaging of human skeletal muscle using TOF-SIMS with bismuth cluster ion as a primary ion source.

摘要: Intramyocellular lipids are of importance in lipid-related diseases. The techniques this field limited because a lack adequate tools for localization various lipids. most usual methods the lipid distribution skeletal muscle histochemistry and fluorescence probes. Different chromatography mass spectrometry have also been used identification. Our aim was to localize spatial their native forms by using static time-of-flight secondary-ion (TOF-SIMS). Human percutaneous biopsies were obtained from middle part lateral vastus right leg healthy adolescents with body index >30. Samples prepared high-pressure freezing, freeze-fracturing freeze-drying, analysed imaging TOF-SIMS equipped Bi3+ cluster ion gun. In positive spectra, we identified phosphocholine, cholesterol, diacylglycerol, phospholipids triacylglycerol. Phosphocholine localized edge fibre, representing sarcoplasma or endomysium. Weak cholesterol signals observed intracellular areas. High diacylglycerol low triacylglycerol signal intensities seen spaces transversal area fibre. negative fatty acids. We co-localization acids which may indicate lipid-storing parts muscle. Thus, can be depict heterogeneous human