Intestinal Obstruction in Germfree Rats

摘要: Introduction PATIENTS with intestinal obstruction who are found to have gangrenous bowel at operation continue a mortality rate of over 20%. 17 Poorly understood toxic substances exude from the ischemic bowel. Although unquestionably important, significant effects bacteria in strangulated remain poorly defined. To explore this problem, natural course was studied series experiments using both conventional and germfree Sprague-Dawley rats. Materials Methods Experiment 1. —A forty 200 250 gm rats normal flora were anesthetized intramuscular sodium pentobarbital, 2.5 mg/100 body weight. With clean but nonsterile instruments techniques, abdominal cavity opened through midline incision. A closed test segment distal ileum created by ligating each end 10 cm section intestine No. 2 black silk