2-D gel membrane protein patterns during fetal rat hepatocyte development.

作者: J RADFORD , P BHATHAL

DOI: 10.1016/0309-1651(87)90093-2

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摘要: Abstract Fetal hepatocytes from rats of 13–20 days gestation were 125I/surface-labelled. Autoradiographs the plasma membrane proteins, analyzed by 2-D gel electrophoresis, compared with those adult and two hepatoma lines. The autoradiographic patterns proteins 13 15 day fetal similar. Between 20 gestation, at least 24 new iodinatable appeared in hepatocytes. Whilst between life, 21 appeared. Two one acidic (MW 25,000) a more basic protein 45,000) present but absent lines less complex than

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