Photohighlighting approaches to access membrane dynamics of the Golgi apparatus.

摘要: By providing quantitative, visual data of live cells, fluorescent protein-based microscopy techniques are furnishing novel insights into the complexities membrane trafficking pathways and organelle dynamics. In this chapter, we describe experimental protocols employing photohighlighting to quantify protein movement out Golgi apparatus, an that serves as central sorting processing station secretory pathway. The methods allow kinetic characteristics Golgi-associated be deciphered, which can help clarify how maintains itself a steady-state structure despite continuous flux cargo passing organelle. guidelines presented in chapter also applied examine dynamics other intracellular systems, elucidating mechanisms for proteins maintained specific organelles and/or circulated destinations within cell.