Imaging the secretory pathway: the past and future impact of live cell optical techniques.
作者： John F. Presley
DOI: 10.1016/J.BBAMCR.2005.04.010
关键词:
摘要: Abstract Classically, the secretory pathway has been studied using a combination of electron microscopic, biochemical and genetic approaches. In last 20 years with arrival molecular biology epitope tagging, fluorescence microscopy become more important than previously. Moreover, common availability Green Fluorescent Protein (GFP) confocal microscopes in 10 years, live cell imaging major experimental approach. This review highlights impact recent introduction single-cell quantitative time-lapse photobleach techniques on study pathway, potential those optical which may play significant future role Golgi apparatus pathway. Particular attention is paid to (Fluorescence Resonance Energy Transfer, Fluorescence Correlation Spectroscopy) can monitor protein–protein interactions living cells.
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