Expression of ACC oxidase genes differs among sex genotypes and sex phases in cucumber.

摘要: Ethylene has been implicated as a sex-determining hormone in cucumber: its exogenous application increases femaleness, and gynoecious genotypes were reported to produce more ethylene. In this study, three full-length ACC oxidase cDNAs isolated from cucumber floral buds. RFLP analysis of population that segregates for the F (femaleness) locus indicated CS-ACO2 is linked at distance 8.7 cM. Expression two genes, CS-ACO3, was monitored flowers, shoot tips leaves different sex genotypes. situ mRNA hybridization patterns tissue- stage-specific expression CS-ACO3 developing flowers. mid-stage female flowers localized nectaries, pistil arrested staminoids, whereas transcript levels accumulated later found placental tissue, ovary staminoids. male petals nectaries expressed both ACO2 strong pollen mature young buds, observed along vascular bundles. Four compared apex leaf. FF had higher but lower ff genotypes; shoot-tip pattern is, therefore, inversely correlated with possibility feedback inhibition mechanism underlying such correlation discussed. The CS-ACO genes studied displayed differential response ethrel treatment organs genotypes, further demonstrating complexity mechanisms controlling ethylene production during development.