Catalases and thioredoxin peroxidase protect Saccharomyces cerevisiae against Ca2+-induced mitochondrial membrane permeabilization and cell death
作者： Alicia J. Kowaltowski , Anibal E. Vercesi , Sue Goo Rhee , Luis E.S. Netto
DOI: 10.1016/S0014-5793(00)01526-X
关键词:
摘要: The involvement of reactive oxygen species in Ca2+-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells which the thioredoxin peroxidase (TPx) gene disrupted and/or catalase inhibited by 3-amino-1,2,4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae were very resistant to Ca2+ inorganic phosphate or t-butyl hydroperoxide-induced permeabilization, but suffered an immediate decrease potential when treated with dithiol binding reagent phenylarsine oxide. In contrast, S. spheroblasts lacking TPx ATZ a potential, generated higher amounts hydrogen peroxide had decreased under these conditions. all cases, could be ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid, dithiothreitol ADP, not cyclosporin A. We conclude that act together, maintaining protecting mitochondria against Ca2+-promoted presents similar characteristics mammalian permeability transition.
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