Thiol enzymes protecting mitochondria against oxidative damage.

摘要: Publisher Summary Mitochondria constantly generate reactive oxygen species (ROS), which can damage their lipids, proteins, DNA, and RNA. The respiratory chain, responsible for a constant generation of ROS within the cell, is located mostly inner mitochondrial membrane. Much oxidative affecting membrane proteins involves thiol oxidation. Thiol oxidation result in impairment secondary to chain inhibition or opening nonselective pore membrane, known as permeability transition pore. These forms impairment, if widespread, significantly affect cellular energy balance cell death. Because are major targets ROS, these organelles contain antioxidant enzymes, capable preventing reversing damage. This chapter reviews techniques used determine protective effects enzymes on mitochondria. Two distinct systems used: (1) treatment isolated mammalian mitochondria with exogenous (2) evaluation S. cerevisae spheroblasts, manipulated remove naturally occurring enzymes.