Characterization of expression of α1-acid glycoprotein gene in Beijing fatty chicken (Gallus gallus).
作者： Chang-Qing LIU , Yu Guo , Shuai LIU , A-Dong BAO , Tao-Feng LU
DOI: 10.3724/SP.J.1005.2009.00620
关键词:
摘要: The specific expression of alpha1-AGP gene in eight different tissues Beijing fatty chicken was investigated by RT-PCR. full-length cDNA inserted into pEGFP-C1 multi-cloning sites to construct recombinant eukaryotic vector pEGFP-alpha1-AGP. lipofectin method used transfect the pEGFP-alpha1-AGP fibroblast cells. open reading frame 612 base pairs length, which expressed higher liver and lung than muscle. This did not express heart kidney. efficiency ranged from 31.3% 47.6% 24, 48, 72 h after transformation. green fluorescence mainly concentrated nucleus. With increase fluorescence, granula observed RT-PCR Western blotting analyses showed that had been integrated genome cell with normal level. In optimized condition, there no significant effect (P>0.05) on apoptosis ratio, positive shape, growth reduplication state comparing control group. research established foundation for further function application transgenic animal cloning.
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