Melittin-induced [Ca2+]i increases and subsequent death in canine renal tubular cells.
作者： SI Liu , HH Cheng , CJ Huang , HC Chang , WC Chen
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摘要: The effect of melittin on cytosolic free Ca(2+) concentration ([Ca(2+)](i)) and viability is largely unknown. This study examined whether alters levels causes Ca(2+)-dependent cell death in Madin-Darby canine kidney (MDCK) cells. [Ca(2+)](i) were measured using the fluorescent dyes fura-2 WST-1 respectively. Melittin at concentrations above 0.5 microM increased a concentration-dependent manner. signal was reduced by 75% removing extracellular Ca(2+). melittin-induced influx also implicated melittin-caused Mn(2+) influx. After pretreatment with 1 thapsigargin (an endoplasmic reticulum pump inhibitor), release inhibited; conversely, abolished thapsigargin-induced release. At 0.5-20 microM, killed cells cytotoxic nearly completely reversed prechelating BAPTA. 0.5-2 caused apoptosis as assessed flow cytometry propidium iodide staining. Collectively, MDCK cells, induced rise causing from space. Furthermore, can cause cytotoxicity
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