Protective effect of human granulocyte colony-stimulating factor on microbial infection in neutropenic mice.

摘要: A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice receiving cyclophosphamide (CPA). severe reduction peripheral blood neutrophils induced 4 days after injection with 200 mg CPA per kg although level normalized rapidly thereafter. When were injected subcutaneously once a day 2.5 micrograms hG-CSF beginning injection, prevented markedly, even later. On other hand, prior infection, weakened resistance intraperitoneal challenge strain Pseudomonas aeruginosa induced. This dose-dependently restored normal by four daily injections hG-CSF. dose 1.0 microgram required complete restoration, did not directly inhibit bacterial growth vitro. In hG-CSF-treated mice, morphologically mature migrated into peritoneal cavities where bacteria inoculated, followed rapid elimination from locality as compared controls. addition, same treatment able protect significantly against systemic caused Serratia marcescens, Escherichia coli, Staphylococcus aureus, Candida albicans. These data show possibility that prophylactic therapy may augment immunocompromised patients infections.