High Prevalence of Rotavirus A in Raw Sewage Samples from Northeast Spain.
作者： Marcelle Silva-Sales , Sandra Martínez-Puchol , Eloy Gonzales-Gustavson , Ayalkibet Hundesa , Rosina Gironès
DOI: 10.3390/V12030318
关键词:
摘要: Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritisworldwide, being a public health threat, as it excreted in large amounts stool and can persist inthe environment for extended periods. In this study, we performed detection of RVA humanadenovirus (HAdV) by TaqMan qPCR assessed circulation genotypes threewastewater treatment plants (WWTPs) between 2015 2016 Catalonia, Spain. wasdetected 90% HAdV 100% WWTP samples, viral loads ranging 3.96 ×104 3.30 × 108 RT-PCR Units/L 9.51 104 1.16 106 genomic copies/L, respectively. RVAVP7 VP4 gene analysis revealed G2, G3, G9, G12, P[4], P[8], P[9] P[10].Nucleotide sequencing (VP6 fragment) showed I1 I2 genotypes, commonlyassociated human, bovine porcine strains. It important to mention that strainsisolated from WWTPs were different those recovered piglets calves living thesame area single sampling 2016. These data highlight importance monitoring watermatrices epidemiology may be useful tool evaluate predict possibleemergence/reemergence uncommon strains region.
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