Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans.
作者： Domenica Farci , Matthew W. Bowler , Francesca Esposito , Sean McSweeney , Enzo Tramontano
关键词:
摘要: The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In present study has been purified and its oligomeric profile characterized by means size exclusion chromatography gel electrophoresis. was found to be organized into three hierarchical orders monomers, stable dimers formed occurrence disulphide bonds, hexamers resulting from combination dimers. structural implications these findings are discussed providing new elements for more integrated model this S-layer.
frontiersin.org
osti.gov
core.ac.uk 
sci-hub.se 参考文章(26)
HERMANN SCHÄGGER, 5 – Blue Native Electrophoresis Membrane Protein Purification and Crystallization (Second Edition)#R##N#A Practical Guide. pp. 105- 130 ,(2003) , 10.1016/B978-012361776-7/50006-1
Robert G. E. Murray, The Family Deinococcaceae Springer, New York, NY. pp. 3732- 3744 ,(1992) , 10.1007/978-1-4757-2191-1_42
Uwe B. Sleytr, Regular arrays of macromolecules on bacterial cell walls: structure, chemistry, assembly, and function. International Review of Cytology-a Survey of Cell Biology. ,vol. 53, pp. 1- 62 ,(1978) , 10.1016/S0074-7696(08)62240-8
Wolfgang BAUMEISTER, Franck KARRENBERG, Reinhard RACHEL, Andreas ENGEL, Beatrice TEN HEGGELER, W. Owen SAXTON, The major cell envelope protein of Micrococcus radiodurans (R1). Structural and chemical characterization. FEBS Journal. ,vol. 125, pp. 535- 544 ,(1982) , 10.1111/J.1432-1033.1982.TB06715.X
M L Faraldo, M A de Pedro, J Berenguer, Cloning and expression in Escherichia coli of the structural gene coding for the monomeric protein of the S layer of Thermus thermophilus HB8. Journal of Bacteriology. ,vol. 173, pp. 5346- 5351 ,(1991) , 10.1128/JB.173.17.5346-5351.1991
M M Faraldo, M A de Pedro, J Berenguer, Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli. Journal of Bacteriology. ,vol. 174, pp. 7458- 7462 ,(1992) , 10.1128/JB.174.22.7458-7462.1992
F. Ferre, P. Clote, DiANNA 1.1: an extension of the DiANNA web server for ternary cysteine classification Nucleic Acids Research. ,vol. 34, pp. 182- 185 ,(2006) , 10.1093/NAR/GKL189
Hermann Schägger, Gebhard von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry. ,vol. 166, pp. 368- 379 ,(1987) , 10.1016/0003-2697(87)90587-2
Margit Sára, Uwe B. Sleytr, S-Layer Proteins Journal of Bacteriology. ,vol. 182, pp. 859- 868 ,(2000) , 10.1128/JB.182.4.859-868.2000
Domenica Farci, Matthew W. Bowler, Joanna Kirkpatrick, Sean McSweeney, Enzo Tramontano, Dario Piano, New features of the cell wall of the radio-resistant bacterium Deinococcus radiodurans. Biochimica et Biophysica Acta. ,vol. 1838, pp. 1978- 1984 ,(2014) , 10.1016/J.BBAMEM.2014.02.014