Effects of antisense misexpression of CFC on downstream flectin protein expression during heart looping.

摘要: Dextral looping of the heart is regulated on multiple levels. In humans, mutations genes CFC and Pitx2/RIEG result in laterality-associated cardiac anomalies. animal models, a common read-out after misexpression laterality direction. Missing these studies how impact downstream morphogenetic processes to coordinate looping. Previously, we showed that Pitx2 indirectly regulates flectin protein by regulating timing expression one field versus other (Linask et al. [2002] Dev. Biol. 246:407-417). To address this question further used reported loss-of-function approach interfere with chick (Schlange [2001] 234:376-389) assaying for during Antisense treatment results abnormal or no Our show regardless sidedness expression, it predominant extracellular matrix dorsal mesocardial folds splanchnic mesoderm apposed foregut wall associated directly Thus, can be experimentally uncoupled from The asymmetry continues maintained secondary