Effect of PDE5 inhibition combined with free oxygen radical scavenger therapy on erectile function in a diabetic animal model.

摘要: Phosphodiesterase (PDE) inhibitors represent an important advance in the treatment of erectile dysfunction (ED). In spite widespread use and generally good efficacy, as a class they remain ineffective 15-57% men. Specific cohorts patients with severe vascular or neurogenic basis to their ED, such diabetic men those who have undergone radical pelvic surgery, demonstrate lower response rates PDE inhibition treatment. We believe that circulating levels nitric oxide (NO) may be enhanced through delivery adequate concentrations free oxygen scavenger molecules vitamin E. Higher NO, theoretically, should produce increased penile blood flow potential for synergistic effect when combined PDE5 inhibitor. With this hypothesis mind, 20 adult male Sprague-Dawley streptozotocin-induced (60 mg/kg i.p.) rats were divided into four therapeutic groups (n=5). Group I--control animals received peanut oil, group II--vitamin E IU/day, III--sildenafil 5 mg/kg/day IV--vitamin IU/day plus sildenafil mg/kg/day, by oral gavage daily 3 weeks. Erectile function was assessed rise intracavernous pressure following cavernous nerve electrostimulation. Penile tissue harvested determine changes morphology including neuronal synthase, smooth muscle alpha-actin endothelial cell integrity. protein content activity measured. Significant increases measured receiving Immunohistochemical staining showed staining. Western blot analysis did not show significant differences between groups. However, higher recorded cohort sildenafil. Vitamin inhibitor meaningful way animal model diabetes. This study indicates means salvaging among are refractory