Improvement of Radiotherapy-Induced Lacrimal Gland Injury by Induced Pluripotent Stem Cell-Derived Conditioned Medium via MDK and Inhibition of the p38/JNK Pathway
作者： Yanqing Zhang , Chenliang Deng , Jiang Qian , Mingui Zhang , Xiaofeng Li
关键词:
摘要: Radiation therapy is the most widely used and effective treatment for orbital tumors, but it causes dry eye due to lacrimal gland damage. Induced pluripotent stem cell-derived conditioned medium (iPSC-CM) has been shown rescue different types of tissue The present study investigated mechanism potential radioprotective effect IPS on gamma-irradiation-induced injury (RILI) in experimental mice. In this study, we found that iPSC-CM ameliorated RILI. markedly decreased radiotherapy induced inflammatory processes, predominantly through suppressing p38/JNK signaling. Further signaling pathway analyses indicated could suppress Akt (Protein Kinase B, PKB) phosphorylation. High levels midkine (MDK) were also be involved regeneration by promoting cell migration proliferation. Thus, our indicates inhibiting or increasing MDK level might a therapeutic target radiation-induced injury.
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R Bessout, A Sémont, C Demarquay, A Charcosset, M Benderitter, N Mathieu, Mesenchymal stem cell therapy induces glucocorticoid synthesis in colonic mucosa and suppresses radiation-activated T cells: new insights into MSC immunomodulation Mucosal Immunology. ,vol. 7, pp. 656- 669 ,(2014) , 10.1038/MI.2013.85
Yong Wang, Joshua Kellner, Lingbo Liu, Daohong Zhou, Inhibition of p38 Mitogen-Activated Protein Kinase Promotes Ex Vivo Hematopoietic Stem Cell Expansion Stem Cells and Development. ,vol. 20, pp. 1143- 1152 ,(2011) , 10.1089/SCD.2010.0413
G.L. Ruskell, Nerve terminals and epithelial cell variety in the human lacrimal gland. Cell and Tissue Research. ,vol. 158, pp. 121- 136 ,(1975) , 10.1007/BF00219955
T A Mitsiadis, T Muramatsu, H Muramatsu, I Thesleff, Midkine (MK), a heparin-binding growth/differentiation factor, is regulated by retinoic acid and epithelial-mesenchymal interactions in the developing mouse tooth, and affects cell proliferation and morphogenesis. Journal of Cell Biology. ,vol. 129, pp. 267- 281 ,(1995) , 10.1083/JCB.129.1.267
Nazir M. Khan, T.B. Poduval, Bilirubin augments radiation injury and leads to increased infection and mortality in mice: molecular mechanisms. Free Radical Biology and Medicine. ,vol. 53, pp. 1152- 1169 ,(2012) , 10.1016/J.FREERADBIOMED.2012.07.007
Masako Ikutomo, Harutoshi Sakakima, Fumiyo Matsuda, Yoshihiro Yoshida, Midkine-deficient mice delayed degeneration and regeneration after skeletal muscle injury Acta Histochemica. ,vol. 116, pp. 319- 326 ,(2014) , 10.1016/J.ACTHIS.2013.08.009
K. Kotenko, B. Moroz, N. Nadezhina, I. Galstyan, I. Eremin, J. Deshevoy, V. Lebedev, T. Slobodina, D. Grinakovskaya, Y. Zhgutov, A. Bushmanov, Successful treatment of localised radiation lesions in rats and humans by mesenchymal stem cell transplantation Radiation Protection Dosimetry. ,vol. 151, pp. 661- 665 ,(2012) , 10.1093/RPD/NCS177
Peng Zhang, Yuqiong Wu, Qinggang Dai, Bing Fang, Lingyong Jiang, p38-MAPK signaling pathway is not involved in osteogenic differentiation during early response of mesenchymal stem cells to continuous mechanical strain Molecular and Cellular Biochemistry. ,vol. 378, pp. 19- 28 ,(2013) , 10.1007/S11010-013-1589-7
Jaroslav Pejchal, Jakub Novotný, Václav Mařák, Jan Österreicher, Aleš Tichý, Jiřina Vávrová, Zuzana Šinkorová, Lenka Zárybnická, Eva Novotná, Jaroslav Chládek, Andrea Babicová, Klára Kubelková, Kamil Kuča, Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation. International Journal of Radiation Biology. ,vol. 88, pp. 348- 358 ,(2012) , 10.3109/09553002.2012.654044
Peter Böhlen, Imre Kovesdi, HBNF and MK, members of a novel gene family of heparin-binding proteins with potential roles in embryogenesis and brain function Progress in Growth Factor Research. ,vol. 3, pp. 143- 157 ,(1991) , 10.1016/S0955-2235(05)80005-5