Purification and characterization of a bovine acetyl low density lipoprotein receptor.

摘要: Abstract The acetyl low density lipoprotein (LDL) receptor is expressed on macrophages and some endothelial cells mediates macrophage-foam cell formation in culture. A 220-kDa LDL binding protein was partially purified from bovine liver membranes used to make a specific monoclonal antibody. The immunoprecipitated by this antibody retained activity, the detect lining sinusoids surface of cultured alveolar macrophages. In human monocytic line THP-1, expression both activity were dramatically induced parallel after differentiation macrophage-like state phorbol ester. ligand specificity, tissue cell-type coinduction data indicated that cell-surface probably endocytosis. protein, which 238,000-fold lung near homogeneity using affinity chromatography, trimer 77-kDa subunits contain asparagine-linked carbohydrate chains.