Vasoactive intestinal peptide (VIP) stimulates rat prostatic epithelial cell proliferation

摘要: BACKGROUND Androgens play a major role in supporting normal growth and functional maintenance the prostate. However, this gland contains an array of neuroendocrine peptides that can regulatory its physiopathology. Among these peptides, one best studied is vasoactive intestinal peptide (VIP), which abundant autonomic nerves surrounding both human rat prostatic acini. This neuropeptide may act through interaction with two types high-affinity receptors, named VPAC1 VPAC2 receptors. Another peptide, pituitary adenylate cyclase-activating (PACAP), interacts receptors same affinity as VIP, but binds higher to PAC1 Human prostate tumors show presence whereas various findings suggest for VIP development. Here we effects on proliferation epithelial cells culture. METHODS We [3H]-thymidine uptake by culture, characterized previously using biomarkers such cytokeratin vimentin. In tested effect PACAP-27 different signaling pathways, cyclic AMP (cAMP) inositol phosphate (IPs). RESULTS The culture were (5,6,8) vimentin positive, indicating was predominantly epithelial. The curves showed followed states growth: quiescent, exponential proliferative, steady state. Cyclic production, not increased PACAP-27, suggests expression and/or primarily. significantly cell bimodal manner, shown dibutyryl (dbcAMP), upon cAMP-dependent. CONCLUSIONS Here, demonstrate [3H]thymidine conceivably activation cyclase. Prostate 47:285–292, 2001. © 2001 Wiley-Liss, Inc.