Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene
作者： Thomas Schödl , Peter Hegemann , Markus Fuhrmann , Markus Heitzer , Amparo Hausherr
DOI: 10.1007/S11103-004-2150-6
关键词:
摘要: For monitoring the expression profile of selected nuclear genes in Chlamydomonas reinhardtii response to altered environmental parameters or during cell cycle, past many RNA protein samples had be taken and analyzed by hybridization immunoblotting. Here we report synthesis a gene that codes for luciferase Renilla reniformis (RLuc) is adapted codon usage C. reinhardtii. This crluc was expressed alone as fusion zeocin resistance ble under control different promoter variants. Luciferase activity monitored living cells, increased with strength paralleled amount protein. Under Lhcb-1 Luc-activity synchronized cultures dependent on dark-light cycle. Additionally, placed Chop-2 measured light conditions. found most pronouced low-light dark conditions, further supporting channelrhodopsin-2 active dark-adapted cells. We conclude reliable tool convenient
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