Luciferase as a Reporter of Gene Activity in Plants

摘要: Reporter gene systems based upon modified luciferase genes isolated from organisms, ranging bacteria to insects, have proven be important tools for plant molecular studies. The biochemical characteristics of these combine very high sensitivity with the ability determine reporter activity non-destructively in vivo, allowing many applications plants that cannot accomplished using any other single system. relative ease situ detection firefly has made it an especially successful screening mutants model genetic system, Arabidopsis thaliana. rapid turnover rate aided characterization promoters and elements are influenced by time-sensitive factors such as circardian rhythm (diurunal cycles), silencing, environmental stresses. a facilitated analysis development synthetic expression. Additionally, different luciferases allowed their use indicators metabolic oxygen levels, well direct vivo protein-protein interaction. various luciferase-based will subject this review.