A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.).

作者: Thomas Cheeran Amal , Palanisamy Karthika , Gurusamy Dhandapani , Subramaniam Selvakumar , Krishnan Vasanth

DOI: 10.1186/S43141-020-00023-Z

关键词:

摘要: Recalcitrant nature is a major constraint for the in vitro regeneration and genetic transformation of leguminous species members. Therefore, an improved horse gram has been developed via planta method, which Agrobacterium strain harboring binary vector pCAMBIA2301 was used transformation. Several factors affecting transformations were put forth viz. cell density, co-cultivation, sonication combined with vacuum infiltration duration optimized. Germinated seeds sonicated infiltrated different densities culture co-cultivated half-strength MS medium 100 μM acetosyringone 48 h. Seedlings washed cefotaxime sowed vermiculite soil maturation. T1 plants subjected to histochemical molecular analysis ensure efficiency. Among various combinations analyzed, maximum efficiency (20.8%) attained 5 min 0.6 optical density culture. It concludes that transgenic plants. This simple efficient method feasible stable expression foreign genes could be beneficial future food security.

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