Assessment of the peripheral benzodiazepine receptors in human gliomas by two methods.

摘要: This study was designed to evaluate the density of peripheral benzodiazepine receptor (PBR) sites as a function tumor malignancy in human gliomas, and compare results obtained with autoradiographic liquid scintillation measurements performed on same tissue specimens. In vitro binding [3H]PK-11195[1-(2-chlorophenyl)-N-methyl-(1-methylpropyl)-3-isog uinoline carboxamide] gliomas radioligand studies revealed significantly higher level (about 3 fold) PBR both low grade high compared normal cortex. The Bmax (mean ± SD) when entire sections were measured by autoradiography, 5.5 0.3 pmol/mg-tissue (n = 5) 1.8 0.9 6), respectively, although it evident that there area hot spots tumors. difference significant (p < 0.05; two-tailed t-test). Similarly, KD values (dissociation constant; nM) between (KD 20.4 1.3 14.3 2.1 different. A site (Bmax) two types also measurements. spot areas which showed most intense [3H]PK-11195 had 24.5 1.0 nM 6.2 0.42 pmol/mg-tissue, 0.05, t-test) than those section measured. data Bmax/KD ratios presented here suggest might be possible differentiate from vivo imaging with11 C-labelled PK-11195.