Cross Protection against Influenza A Virus by Yeast-Expressed Heterologous Tandem Repeat M2 Extracellular Proteins
作者： Yu-Na Lee , Min-Chul Kim , Young-Tae Lee , Hye Suk Hwang , Jongsang Lee
DOI: 10.1371/JOURNAL.PONE.0137822
关键词:
摘要: The influenza M2 ectodomain (M2e) is well conserved across human A subtypes, but there are few residue changes among avian and swine origin viruses. We expressed a tandem repeat construct of heterologous M2e sequences (M2e5x) derived from human, swine, viruses using the yeast expression system. Intramuscular immunization mice with AS04-adjuvanted M2e5x protein vaccines was effective in inducing M2e-specific antibodies reactive to peptide native proteins on infected cells or virus, mucosal systemic memory cellular immune responses, cross-protection against H3N2 virus. Importantly, sera were found confer protection different subtypes H1N1 H5N1 naive mice. Also, M2e5x-immune complexes virus-infected stimulated macrophages secrete cytokines via Fc receptors, indicating possible mechanism protection. present study provides evidence that produced could be developed as potential universal vaccine.
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Sabine Neirynck, Tom Deroo, Xavier Saelens, Peter Vanlandschoot, Willy Min Jou, Walter Fiers, A universal influenza A vaccine based on the extracellular domain of the M2 protein. Nature Medicine. ,vol. 5, pp. 1157- 1163 ,(1999) , 10.1038/13484
J J Treanor, E L Tierney, S L Zebedee, R A Lamb, B R Murphy, Passively transferred monoclonal antibody to the M2 protein inhibits influenza A virus replication in mice. Journal of Virology. ,vol. 64, pp. 1375- 1377 ,(1990) , 10.1128/JVI.64.3.1375-1377.1990
Yumi Hashimoto, Takeshi Moki, Takenori Takizawa, Akiko Shiratsuchi, Yoshinobu Nakanishi, Evidence for Phagocytosis of Influenza Virus-Infected, Apoptotic Cells by Neutrophils and Macrophages in Mice Journal of Immunology. ,vol. 178, pp. 2448- 2457 ,(2007) , 10.4049/JIMMUNOL.178.4.2448
Karim El Bakkouri, Francis Descamps, Marina De Filette, Anouk Smet, Els Festjens, Ashley Birkett, Nico Van Rooijen, Sjef Verbeek, Walter Fiers, Xavier Saelens, Universal Vaccine Based on Ectodomain of Matrix Protein 2 of Influenza A: Fc Receptors and Alveolar Macrophages Mediate Protection The Journal of Immunology. ,vol. 186, pp. 1022- 1031 ,(2011) , 10.4049/JIMMUNOL.0902147
T Ito, O T Gorman, Y Kawaoka, W J Bean, R G Webster, Evolutionary analysis of the influenza A virus M gene with comparison of the M1 and M2 proteins. Journal of Virology. ,vol. 65, pp. 5491- 5498 ,(1991) , 10.1128/JVI.65.10.5491-5498.1991
Andrea Jegerlehner, Nicole Schmitz, Tazio Storni, Martin F. Bachmann, Influenza A Vaccine Based on the Extracellular Domain of M2: Weak Protection Mediated via Antibody-Dependent NK Cell Activity Journal of Immunology. ,vol. 172, pp. 5598- 5605 ,(2004) , 10.4049/JIMMUNOL.172.9.5598
Paul P. Heinen, Frans A. Rijsewijk, Els A. de Boer-Luijtze, André T. J. Bianchi, Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus Journal of General Virology. ,vol. 83, pp. 1851- 1859 ,(2002) , 10.1099/0022-1317-83-8-1851
Manon M.J. Cox, Jason R. Hollister, FluBlok, a next generation influenza vaccine manufactured in insect cells. Biologicals. ,vol. 37, pp. 182- 189 ,(2009) , 10.1016/J.BIOLOGICALS.2009.02.014
Jae-Min Song, Jaber Hossain, Dae-Goon Yoo, Aleksandr S Lipatov, C Todd Davis, Fu-Shi Quan, Li-Mei Chen, Robert J Hogan, Ruben O Donis, Richard W Compans, Sang-Moo Kang, None, Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles Virology. ,vol. 405, pp. 165- 175 ,(2010) , 10.1016/J.VIROL.2010.05.034
M. K. L. MacLeod, A. S. McKee, A. David, J. Wang, R. Mason, J. W. Kappler, P. Marrack, Vaccine adjuvants aluminum and monophosphoryl lipid A provide distinct signals to generate protective cytotoxic memory CD8 T cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 7914- 7919 ,(2011) , 10.1073/PNAS.1104588108