Interleukin 17–Producing γδT Cells Promote Hepatic Regeneration in Mice

摘要: Background & Aims Subsets of leukocytes synergize with regenerative growth factors to promote hepatic regeneration. γδT cells are early responders inflammation-induced injury in a number contexts. We investigated the role regeneration using mice disruptions Tcrd (encodes T-cell receptor δ chain) and Clec7a C-type lectin domain family 7 member a, also known as DECTIN1 ). Methods performed partial hepatectomies on wild-type C57BL/6, CD45.1, −/− , or mice. Cells were isolated from livers patients via mechanical enzymatic digestion. purified by fluorescence-activated cell sorting. Results In mice, hepatectomy up-regulated expression CCL20 ligands Dectin-1, which was associated recruitment activation their increased production interleukin (IL)-17 cytokines. Recruited induced IL-6 antigen-presenting suppressed interferon gamma natural killer T cells, promoting hepatocyte proliferation. Absence IL-17–producing deletion Dectin-1 prevented development phenotypes subsets innate immune cells. This slowed liver reduced cycle regulators. Conversely, exogenous administration IL-17 cytokines promoted More broadly, we found that required for inflammatory responses mediated Dectin-1. Conclusions regulate producing IL-22 IL-17, have direct mitogenic effects hepatocytes phenotype leukocytes, respectively. ligation is