Delivery of small interfering RNA by peptide-targeted mesoporous silica nanoparticle-supported lipid bilayers

摘要: The therapeutic potential of small interfering RNAs (siRNAs) is severely limited by the availability delivery platforms that protect siRNA from degradation, deliver it to target cell with high specificity and efficiency, promote its endosomal escape cytosolic dispersion. Here we report mesoporous silica nanoparticle-supported lipid bilayers (or "protocells") exhibit multiple properties overcome many limitations existing platforms. Protocells have a 10- 100-fold greater capacity for than corresponding nanoparticles are markedly more stable when incubated under physiological conditions. loaded cocktail siRNAs bind cells in manner dependent on presence an appropriate targeting peptide and, through endocytic pathway followed disruption, silencing nucleotides cytoplasm. expression each genes targeted was shown be repressed at protein level, resulting potent induction growth arrest apoptosis. Incubation control lack antigen recognized siRNA-loaded protocells induced neither repression nor apoptosis, indicating precise cytotoxic activity. In terms loading capacity, capabilities, potency action, provide unique attributes as platform oligonucleotides.