Co-immobilization of adhesive peptides and VEGF within a dextran-based coating for vascular applications.

摘要: Abstract Multifunctional constructs providing a proper environment for adhesion and growth of selected cell types are needed most tissue engineering regenerative medicine applications. In this context, vinylsulfone (VS)-modified dextran was proposed as matrix featuring low-fouling properties well multiple versatile moieties. The displayed VS groups could indeed react with thiol, amine or hydroxyl groups, be it surface grafting, crosslinking subsequent tethering biomolecules. the present study, library dextran-VS produced, grafted to aminated substrates characterized in terms degree modification (%VS), cell-repelling potential oriented grafting cysteine-tagged peptides. As bioactive coating vascular implants, ECM peptides (e.g. RGD) endothelial factor (VEGF) were co-immobilized on one suitable (%VS =  ca. 50% saccharides units). Both RGD VEGF efficiently tethered at high densities ( 1 nmol/cm 2 50 fmol/cm , respectively), able promote proliferation. latter enhanced same extent soluble proved selective cells over smooth muscle cells. Altogether, biomolecules incorporated into construct, while maintaining their respective biological activity. Statement Significance This work addresses need multifunctional coatings response inherent many applications, instance, graft. More specifically, dextrans first generated through (VS) modification. Thoroughly provided an ideal platform unbiased study covalently Considering that processes such healing angiogenesis require factors acting synergistically, then adhesive peptide within our relevant strategy orientation specificity. both proliferative cues construct additive, if not synergetic, effects.