DNA profiling of Acorus calamus chemotypes differing in essential oil composition.

摘要: The phylogenetic relationship of Acorus gramineus and three types calamus was analyzed by comparing the 700 bp sequence a 5S-rRNA gene spacer region. Although there no intra-specific variation in essential oil profile A. which contained phenylpropanoid (Z-asarone) as predominant constituent, classified into two chemotypes: chemotype A Z-asarone is major constituent B sesquiterpenoids predominantly. An intermediate type (M) these chemotypes various ratios also observable. NJ tree constructed based on sequences revealed that clearly distinguished from any predicted region data correlated well with pattern calamus.