Forskolin activation of adenylate cyclase in vivo stimulates nerve regeneration

摘要: The previous demonstration of an increase and redistribution adenylate cyclase activity in injured peripheral nerve1 suggests that neuronal cyclic AMP concentration could play a role nerve regeneration. We report our finding accumulating was translated into twofold the regenerating stump, coincident with initiation elongation regenerative sprouts. sought to magnify regeneration by using forskolin, robust activator cyclase2, produce additional situ. Forskolin vitro produced approximately 40-fold greater elevation than equimolar (10−5) isoprenaline. Moreover, elevated persisted for at least 60 min continued presence forskolin. Daily injection forskolin dorsal lymph sac Rana pipiens, or delivery through implanted osmotic pump sustained 40% rate sensory freeze-lesioned sciatic nerves. conclude and, presumably, activation appropriate protein kinases stimulates growth following trauma.