Carbon concentration and oxygen availability affect lipid and carotenoid production by carob pulp syrup-grown Rhodosporidium toruloides NCYC 921
作者： Teresa Margarida Parreira , Claudia Freitas , Alberto Reis , José Roseiro , Teresa Lopes da Silva
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摘要: The simultaneous effect of oxygen availability and carbon source concentration on yeast lipid carotenoid production has never been studied before. In this work, a Doehlert distribution design was used to study the Rhodosporidium toruloides NCYC 921 production. A cheap industrial byproduct as (carob pulp syrup). total sugar 106.3 g/L medium volume 0.120 L induced highest fatty acid productivities (4.60 μg/Lh 0.029 g/Lh, respectively). Flow cytometry assess stress response under different cultivation conditions. proportion cells with permeabilised membrane (>20%) when cultivations were carried out at (130.0 g/L) or culture reached minimum final pH (4.60). results showed that had positive influence biomass content, while little concentration, but slight content. Regarding acids, two factors negative impact synthesis these compounds.
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